賽多利斯(百得)mLINE PCR 移液器體驗套裝
mLINE®--更加輕便的移液承諾
體驗套裝產(chǎn)品編號:725660
mLINE 0.5-10 ul 手動單道移液器
mLINE 10-100 ul 手動單道移液器
mLINE 100-1000 ul 手動單道移液器
10ul 濾芯吸頭盒裝 預(yù)消毒
120ul 濾芯吸頭盒裝 預(yù)消毒
1000ul 濾芯吸頭盒裝 預(yù)消毒
移液器掛鉤 ,3個
開帽器/校準(zhǔn)工具
3個彩色識別帽
冷凝筆 (黑色)
鋁制冷卻架可容納12個微型試管
上海攸茜 http://www.shyouqian.com/主營產(chǎn)品有:移液器(移液器吸頭,移液器架)、低溫冰箱、冷凍干燥機(jī)(凍干機(jī))、雪花制冰機(jī) 、真空泵、高壓滅菌鍋、恒溫?fù)u床、CO2培養(yǎng)箱、干燥箱、生物安全柜、潔凈工作臺、水份檢測儀、粘度計、電位滴定儀、PH計(電極,緩沖液)、電導(dǎo)率儀 (電極、緩沖液)、比色計、旋轉(zhuǎn)蒸發(fā)儀、攪拌器(磁力攪拌器,頂置式攪拌器,混合儀,分散機(jī),均質(zhì)器)、離心機(jī)、分光光度計、實驗室分析天平等實驗室常用設(shè)備;產(chǎn)品品牌保證,服務(wù)更周全。
核心優(yōu)勢:
型號 | 外部尺寸 (mm) (長 x 寬 x 高) | 內(nèi)部尺寸 (mm) (長 x 寬 x 高) | 層流速率 | 電源電壓 |
PCR-3A1 | 1035 x 617 x 950 mm (40.7" x 24.3" x 37.4") | 935 x 538 x 550 mm (36.8" x 21.2" x 21.7") | 0.30 m/s (60 fpm) | 230 VAC 50 Hz |
PCR-4A1 | 1340 x 617 x 950 mm (52.8" x 24.3"x 37.4") | 1240 x 538 x 550 mm (48.8" x 21.2" x 21.7”) | 230 VAC 50 Hz |
NEST PCR96孔板
產(chǎn)品編號 | 產(chǎn)品描述 | 包裝規(guī)格 |
402101 | 0.15ml PCR96孔板,無裙邊矮管,透明 | 25塊/盒,4盒/箱 |
402401 | 0.15ml PCR96孔板,半裙邊矮管,透明 | 25塊/盒,4盒/箱 |
402001 | 0.2ml PCR96孔板,無裙邊高管,透明 | 25塊/盒,4盒/箱 |
402201 | 0.2ml PCR96孔板,無裙邊高孔緣,透明 | 25塊/盒,4盒/箱 |
402301 | 0.2ml PCR96孔板,半裙邊高管,透明 | 25塊/盒,4盒/箱 |
402111 | 0.15ml PCR96孔板,無裙邊矮管,白色 | 25塊/盒,4盒/箱 |
402411 | 0.15ml PCR96孔板,半裙邊矮管,白色 | 25塊/盒,4盒/箱 |
402011 | 0.2ml PCR96孔板,無裙邊高管,白色 | 25塊/盒,4盒/箱 |
402211 | 0.2ml PCR96孔板,無裙邊高孔緣,白色 | 25塊/盒,4盒/箱 |
402311 | 0.2ml PCR96孔板,半裙邊高管,白色 | 25塊/盒,4盒/箱 |
產(chǎn)品編號 | 產(chǎn)品名稱 | 包裝規(guī)格 |
401001 | 0.2ml PCR管,平蓋,透明 | 1000個/盒,10盒/箱 |
403101 | 0.15ml PCR8聯(lián)排管(無蓋),矮管,透明 | 120條/盒,10盒/箱 |
403111 | 0.15ml PCR8聯(lián)排管(無蓋),矮管,白色 | 120條/盒,10盒/箱 |
403001 | 0.2ml PCR8聯(lián)排管(無蓋),高管,透明 | 120條/盒,10盒/箱 |
403011 | 0.2ml PCR8聯(lián)排管(無蓋),高管,白色 | 120條/盒,10盒/箱 |
406011 | PCR8聯(lián)排蓋,平蓋 | 120條/盒,10盒/箱 |
406111 | PCR8聯(lián)排蓋,凸蓋 | 120條/盒,10盒/箱 |
407001 | PCR管架,96孔(8×12),單獨蓋 | 5個/包,25個/箱 |
407101 | PCR管架,96孔(8×12),5個架一蓋 | 5個/包,25個/箱 |
樣品容量 | 96 |
反應(yīng)體積 | 1–50µl(推薦 10–25 µl) |
溫度范圍 | 0-100℃ |
激發(fā)/檢測波長范圍 | 450–580 nm |
熔解曲線分辨率 | 0.1˚C |
多重檢測能力 | 每孔最多2個基因 |
靈敏度 | 1拷貝人基因組 DNA |
動態(tài)范圍 | 10個數(shù)量級 |
尺寸(mm) | 330×460×360 |
重量(KG) | 21 |
ReverTra Ace® qPCR RT Kit | FSQ-101 | 200次份 × 1 |
人可溶性血管內(nèi)皮細(xì)胞蛋白C受體(sEPCR)ELISA檢測試劑盒
本試劑盒僅供研究使用。
人可溶性血管內(nèi)皮細(xì)胞蛋白C受體(sEPCR)ELISA檢測試劑盒實驗原理
本試劑盒應(yīng)用雙抗體夾心法測定標(biāo)本中人可溶性血管內(nèi)皮細(xì)胞蛋白C受體(sEPCR)水平。用純化的人可溶性血管內(nèi)皮細(xì)胞蛋白C受體(sEPCR)抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入sEPCR,再與HRP標(biāo)記的sEPCR抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過徹底洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的sEPCR呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(OD值),通過標(biāo)準(zhǔn)曲線計算樣品中人可溶性血管內(nèi)皮細(xì)胞蛋白C受體(sEPCR)濃度。
人可溶性血管內(nèi)皮細(xì)胞蛋白C受體(sEPCR)ELISA檢測試劑盒試劑盒組成
1 | 30倍濃縮洗滌液 | 20ml×1瓶 | 7 | 終止液 | 6ml×1瓶 |
2 | 酶標(biāo)試劑 | 6ml×1瓶 | 8 | 標(biāo)準(zhǔn)品(160μg/L) | 0.5ml×1瓶 |
3 | 酶標(biāo)包被板 | 12孔×8條 | 9 | 標(biāo)準(zhǔn)品稀釋液 | 1.5ml×1瓶 |
4 | 樣品稀釋液 | 6ml×1瓶 | 10 | 說明書 | 1份 |
5 | 顯色劑A液 | 6ml×1瓶 | 11 | 封板膜 | 2張 |
6 | 顯色劑B液 | 6ml×1/瓶 | 12 | 密封袋 | 1個 |
標(biāo)本要求
1.標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實驗。若不能馬上進(jìn)行試驗,可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
2.不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
人可溶性血管內(nèi)皮細(xì)胞蛋白C受體(sEPCR)ELISA檢測試劑盒操作步驟
1. 標(biāo)準(zhǔn)品的稀釋:本試劑盒提供原倍標(biāo)準(zhǔn)品一支,用戶可按照下列圖表在小試管中進(jìn)行稀釋。
80μg/L | 5號標(biāo)準(zhǔn)品 | 150μl的原倍標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液 |
40μg/L | 4號標(biāo)準(zhǔn)品 | 150μl的5號標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液 |
20μg/L | 3號標(biāo)準(zhǔn)品 | 150μl的4號標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液 |
10μg/L | 2號標(biāo)準(zhǔn)品 | 150μl的3號標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液 |
5μg/L | 1號標(biāo)準(zhǔn)品 | 150μl的2號標(biāo)準(zhǔn)品加入150μl標(biāo)準(zhǔn)品稀釋液 |
2. 加樣:分別設(shè)空白孔(空白對照孔不加樣品及酶標(biāo)試劑,其余各步操作相同)、標(biāo)準(zhǔn)孔、待測樣品孔。在酶標(biāo)包被板上標(biāo)準(zhǔn)品準(zhǔn)確加樣50μl,待測樣品孔中先加樣品稀釋液40μl,然后再加待測樣品10μl(樣品最終稀釋度為5倍)。加樣將樣品加于酶標(biāo)板孔底部,盡量不觸及孔壁,輕輕晃動混勻。
3. 溫育:用封板膜封板后置37℃溫育30分鐘。
4. 配液:將30倍濃縮洗滌液用蒸餾水30倍稀釋后備用
5. 洗滌:小心揭掉封板膜,棄去液體,甩干,每孔加滿洗滌液,靜置30秒后棄去,如此重復(fù)5次,拍干。
6. 加酶:每孔加入酶標(biāo)試劑50μl,空白孔除外。
7. 溫育:操作同3。
8. 洗滌:操作同5。
9. 顯色:每孔先加入顯色劑A50μl,再加入顯色劑B50μl,輕輕震蕩混勻,37℃避光顯色15分鐘.
10. 終止:每孔加終止液50μl,終止反應(yīng)(此時藍(lán)色立轉(zhuǎn)黃色)。
11. 測定:以空白空調(diào)零,450nm波長依序測量各孔的吸光度(OD值)。 測定應(yīng)在加終止液后15分鐘以內(nèi)進(jìn)行。
操作程序總結(jié):
計算
以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度;再乘以稀釋倍數(shù);或用標(biāo)準(zhǔn)物的濃度與OD值計算出標(biāo)準(zhǔn)曲線的直線回歸方程式,將樣品的OD值代入方程式,計算出樣品濃度,再乘以稀釋倍數(shù),即為樣品的實際濃度。
注意事項
1.試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開封后如未用完,板條應(yīng)裝入密封袋中保存。
2.濃洗滌液可能會有結(jié)晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結(jié)果。
3.各步加樣均應(yīng)使用加樣器,并經(jīng)常校對其準(zhǔn)確性,以避免試驗誤差。一次加樣時間最好控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排槍加樣。
4. 請每次測定的同時做標(biāo)準(zhǔn)曲線,最好做復(fù)孔。如標(biāo)本中待測物質(zhì)含量過高(樣本OD值大于標(biāo)準(zhǔn)品孔第一孔的OD值),請先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測定,計算時請最后乘以總稀釋倍數(shù)(×n×5)。
5. 封板膜只限一次性使用,以避免交叉污染。
6.底物請避光保存。
7.嚴(yán)格按照說明書的操作進(jìn)行,試驗結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).
8.所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。
9.本試劑不同批號組分不得混用。
10. 如與英文說明書有異,以英文說明書為準(zhǔn)。
保存條件及有效期
1.試劑盒保存:;2-8℃。
2.有效期:6個月
Pkg of 25, clear shell/clear well PCR plate to fit most instruments, rigid 2-component design
Hard-Shell 96-Well Semi-Skirted PCR Plates are specifically designed to withstand the stresses of thermal cycling and robotic handling. The patented* two-component design provides superior stability and flatness, allowing precise positioning for automation. The full-height (20.75 mm) wells fit most thermal cyclers, real-time PCR detection systems, and DNA sequencers.
Key Features and Benefits
· Reaction volumes of 5–125 µl (350 µl maximum)
· Extremely uniform wells reduce well-to-well variability in optical assays, such as those used in real-time PCR
· Warp-free half-height skirt for improved robotic handling and labeling surface
· Black lettering for easy well identification
· Process sampling and testing to ensure plates are free of DNase, RNase, and human DNA
Options
· White-well option for increased fluorescent signal strength
· Color-coded skirt option with clear wells
· Low-cost, user-readable bar code option for convenient database tracking
Plate Dimensions
| |
Microplate Dimensions | Hard-Shell® High-Profile 96-Well Semi-Skirted PCR Plates (HSS-9xxx) |
Length at top plane | 127.76 mm |
Width at top plane | 85.47 mm |
Height overall | 20.75 mm |
Well depth | 19.85 mm |
Well diameter at opening | 5.50 mm |
Well diameter at bottom of conical section | 2.69 mm |
Well volume | 350 μl |
Well spacing | 9.00 mm |
Well angle | 17.5° |
Well offset | |
Left edge to well A1 | 14.37 mm |
Top edge to well A1 | 11.23 mm |
Left edge to H12 | 113.37 mm |
Top edge to H12 | 74.23 mm |
Skirt format | Semi-skirted |
Well/tube profile | High |
Barcoded PCR plate | No |
Hard-Shell®96孔PCR板,高外形,半裙邊,綠色/透明#HSS9641
Hard-Shell 96-Well Semi-Skirted PCR Plates are specifically designed to withstand the stresses of thermal cycling and robotic handling. The patented* two-component design provides superior stability and flatness, allowing precise positioning for automation. The full-height (20.75 mm) wells fit most thermal cyclers, real-time PCR detection systems, and DNA sequencers.
Key Features and Benefits
· Reaction volumes of 5–125 µl (350 µl maximum)
· Extremely uniform wells reduce well-to-well variability in optical assays, such as those used in real-time PCR
· Warp-free half-height skirt for improved robotic handling and labeling surface
· Black lettering for easy well identification
· Process sampling and testing to ensure plates are free of DNase, RNase, and human DNA
Options
· White-well option for increased fluorescent signal strength
· Color-coded skirt option with clear wells
· Low-cost, user-readable bar code option for convenient database tracking
Plate Dimensions
| |
Microplate Dimensions | Hard-Shell® High-Profile 96-Well Semi-Skirted PCR Plates (HSS-9xxx) |
Length at top plane | 127.76 mm |
Width at top plane | 85.47 mm |
Height overall | 20.75 mm |
Well depth | 19.85 mm |
Well diameter at opening | 5.50 mm |
Well diameter at bottom of conical section | 2.69 mm |
Well volume | 350 μl |
Well spacing | 9.00 mm |
Well angle | 17.5° |
Well offset | |
Left edge to well A1 | 14.37 mm |
Top edge to well A1 | 11.23 mm |
Left edge to H12 | 113.37 mm |
Top edge to H12 | 74.23 mm |
Well/tube format | 96-well |
Well/tube profile | High |
Skirt format | Semi-skirted |
Barcoded PCR plate | No |
Compatible instruments, Bio-Rad | CFX96™, 1000-series, DNA Engine Opticon® series, Chromo4™, PTC-100®, DNA Engine® series |
Compatible instruments, Eppendorf | Mastercycler series |
Well/tube color | Clear |
,高位半裙型黑色白色HSS9665 Hard-Shell®96孔PCR板
Pkg of 25, black shell/white well PCR plate for higher fluorescent signals, rigid 2-component design
Hard-Shell 96-Well Semi-Skirted PCR Plates are specifically designed to withstand the stresses of thermal cycling and robotic handling. The patented* two-component design provides superior stability and flatness, allowing precise positioning for automation. The full-height (20.75 mm) wells fit most thermal cyclers, real-time PCR detection systems, and DNA sequencers.
Key Features and Benefits
· Reaction volumes of 5–125 µl (350 µl maximum)
· Extremely uniform wells reduce well-to-well variability in optical assays, such as those used in real-time PCR
· Warp-free half-height skirt for improved robotic handling and labeling surface
· Black lettering for easy well identification
· Process sampling and testing to ensure plates are free of DNase, RNase, and human DNA
Options
· White-well option for increased fluorescent signal strength
· Color-coded skirt option with clear wells
· Low-cost, user-readable bar code option for convenient database tracking
Plate Dimensions
| |
Microplate Dimensions | Hard-Shell® High-Profile 96-Well Semi-Skirted PCR Plates (HSS-9xxx) |
Length at top plane | 127.76 mm |
Width at top plane | 85.47 mm |
Height overall | 20.75 mm |
Well depth | 19.85 mm |
Well diameter at opening | 5.50 mm |
Well diameter at bottom of conical section | 2.69 mm |
Well volume | 350 μl |
Well spacing | 9.00 mm |
Well angle | 17.5° |
Well offset | |
Left edge to well A1 | 14.37 mm |
Top edge to well A1 | 11.23 mm |
Left edge to H12 | 113.37 mm |
Top edge to H12 | 74.23 mm |
* Hard-Shell plates are covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers 7,347,977; 6,340,589; and 6,528,302.
Well/tube profile | High |
Skirt format | Semi-skirted |
Barcoded PCR plate | No |
交貨時間:款到發(fā)貨。
運輸方式:供方負(fù)責(zé)免費送貨上門。
質(zhì)量標(biāo)準(zhǔn):按宣傳資料、產(chǎn)品說明書所列相關(guān)技術(shù)參數(shù)進(jìn)行驗貨,應(yīng)包括所有配件及產(chǎn)品購買時所附物品及資料。如因運輸造成的貨物損壞,由供方與運輸方協(xié)調(diào)解決,需方不承擔(dān)由此產(chǎn)生的損失。
付款方式:需方一次100%付清貨款,含運費。